- $395
- Leading Biology
- AMM00922G
- 2025年09月22日
- IF, FC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Mouse / IgG1, kappa
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human
- 库存：
100
- 宿主：
Mouse
- 应用范围：
IF, FC
- 规格：
50 ug
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文献和实验Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
with this for 30min to 1 hr. 5. Primary incubation. Make a solution of 1℃ antibody in blotto (or BSA/TBS/Tween). Need only ~5 mls or less for a modest size blot (14X8 cm). Antibody concentration should be optimized; a general guidline is ~1:2000 dilution for a red
Staining protocols for placental explant cultures
there is less likelihood of damaging the culture during processing. Incubate overnight (at least) at 4°C in blocking solution (4% BSA/PBS). Include 0.02% sodium azide if the plates are to be kept longer. Incubate in 1st antibody for 30 min at room temperature. Ideally
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