- $395
- Leading Biology
- AMM00112G
- 2025年09月21日
- WB, IHC, IF, FC, IP, E
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human, Mouse, Rat
- 库存：
100
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF, FC, IP, E
- 靶点：
Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
- 规格：
50 ug
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文献和实验ELISA Procedure for Measuring Serum Antibody Titer
assays are the most appropriate for measuring the titer of the antisera you have generated. ELISA Procedure for Measuring Serum Antibody Titer In this type of ELISA (Enzyme-Linked Immunosorbant Assay), the antigen (peptide or protein) is bound
RUVKUN Antibody Staining Protocol revised 10/29/90
: 11. Can preincubate in Buffer A (1% BSA) at RT for a few hours. 12. Spin down worms gently and incubate with 1 in Buffer A (30 ul affinity purified anti-lin-14 antibody -preadsorbed with 292 vector protein- straight or diluted 1:1 or 1:2) for 24
antibody will be used for detection. ) Wash wells twice with PBS. Add 50 m L of the antigen solution to the wells (the antigen solution should be titrated). All dilutions should be done in the blocking buffer (3% BSA/PBS with 0.02% sodium azide
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