- ￥1375 - 5000
- Leading Biology
- AMM07666G
- 美国
- 2025年08月28日
- WB, IHC-P, FC
- Rabbit
- H, M
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
Ras-related GTP-binding protein D, Rag D, RagD, RRAGD (<a href="http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=19903" target="_blank">HGNC:19903</a>)
- 浓度：
Affinity Purification
- 应用范围：
WB, IHC-P, FC
- 宿主：
Rabbit
- 适应物种：
H, M
- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 克隆性：
Polyclonal
- 保存条件：
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
- 形态：
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
- 免疫原：
This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD.
- 规格：
400 µl/200 µl/100 µl/50 µl
| 规格： | 400 µl | 产品价格： | ￥5000.0 |
|---|---|---|---|
| 规格： | 200 µl | 产品价格： | ￥3250.0 |
| 规格： | 100 µl | 产品价格： | ￥2250.0 |
| 规格： | 50 µl | 产品价格： | ￥1375.0 |
细胞定位：Cytoplasm. Nucleus. Lysosome Note=Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA (PubMed:11073942).
功能：Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed:20381137, PubMed:24095279). Forms heterodimeric Rag complexes with RRAGA or RRAGB and cycles between an inactive GTP-bound and an active GDP-bound form (PubMed:24095279). In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB (PubMed:20381137, PubMed:24095279). This is a crucial step in the activation of the TOR signaling cascade by amino acids (PubMed:20381137, PubMed:24095279).
稀释方法：WB--1:1000 IHC-P--1:100~500 FC--1:10~50
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文献和实验Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
chamber, at RT overnight . Rabbit anti BCL-6 (Santa Cruz Biotechnology, Santa Cruz, CA: Bcl-6 (N-3): sc-858, use at 0.5 µg/ml) 5- Wash twice in TBS-T. 6- add the biotin-conjugated secondary antibody (100 to 200 µl) and incubate for 45 min
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection
, at RT overnight . Rabbit anti BCL-6 (Santa Cruz Biotechnology, Santa Cruz, CA: Bcl-6 (N-3): sc-858, use at 0.5 µg/ml) 5- Wash twice in TBS-T. 6- add the biotin-conjugated secondary antibody (100 to 200 µl) and incubate for 45 min
with those with a postgerminal center/activated profile. Microarray technology may not be available in many diagnostic laboratories, and antibody-based methods are much simpler and cheaper and are therefore more applicable to the routine setting. Immunocytochemistry
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