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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
Fructosamine-3-kinase, 271-, FN3K
- 浓度:
Affinity Purification
- 应用范围:
WB
- 宿主:
Mouse
- 适应物种:
H
- 保质期:
1-2年
- 供应商:
安诺伦(北京)生物科技有限公司
- 克隆性:
Monoclonal
- 保存条件:
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 形态:
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
- 免疫原:
Purified His-tagged FN3K protein was used to produced this monoclonal antibody.
- 规格:
400 µl/200 µl/100 µl/50 µl
| 规格: | 400 µl | 产品价格: | ¥5000.0 |
|---|---|---|---|
| 规格: | 200 µl | 产品价格: | ¥3250.0 |
| 规格: | 100 µl | 产品价格: | ¥2250.0 |
| 规格: | 50 µl | 产品价格: | ¥1375.0 |
功能:Fructosamine-3-kinase involved in protein deglycation by mediating phosphorylation of fructoselysine residues on glycated proteins, to generate fructoselysine-3 phosphate (PubMed:11016445, PubMed:11522682, PubMed:11975663). Fructoselysine-3 phosphate adducts are unstable and decompose under physiological conditions (PubMed:11522682, PubMed:11975663). Involved in intracellular deglycation in erythrocytes (PubMed:11975663). Involved in the response to oxidative stress by mediating deglycation of NFE2L2/NRF2, glycation impairing NFE2L2/NRF2 function (By similarity). Also able to phosphorylate psicosamines and ribulosamines (PubMed:14633848).
总结:Tissue Location: Widely expressed (PubMed:11522682). Expressed in erythrocytes (PubMed:11016445).
稀释方法:WB--1:1000~8000
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文献和实验Purification of Antiserum or Ascites by Protein A/G Chromatography
PreparationTo prepare 1 liter of TBS (50 mM Tris-HCl, pH 7.4; 150 mM NaCl; 0.05% sodium azide) add the following to 800ml of distilled water:Ⅰ6.06 g of Tris baseⅡ8.77 g of NaClⅢ 10ml of 5% sodium azide stock solution (Do not add if the antibody will used
to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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