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- 详细信息
- 文献和实验
- 技术资料
- 库存:
261
- 英文名:
Sulfosuccinimidyl-6-(biotinamido)hexanoate
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冷冻(-20℃)
- 规格:
100mg
特别提示:包括Sulfo-NHS-LC-Biotin在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:Sulfo-NHS-LC-Biotin
英文名称:Sulfosuccinimidyl-6-(biotinamido)hexanoate
产品货号:GS4321
产品规格:100mg
分子量:556.6
储存条件:冷冻(-20℃)
概述
Sulfo-NHS-LC-Biotin(sulfosuccinimidyl-6-[biotin-amido]hexanoate)enables simple and efficient labeling of antibodies,
proteins and any other primary amine-containing molecules.Specific labeling of cell surface proteins is another common
application for this water-soluble and membrane impermeable reagent.
Biotin is a vitamin that binds with high affinity to avidin and streptavidin proteins.Because it is small(244 Da),biotin can be
conjugated to many proteins without altering their biological activities.Labeled proteins can be purified from unlabeled
proteins using immobilized streptavidin and avidin and detected in ELISA,dot blot or Western blot applications using
streptavidin or avidin-conjugated probes.
应用
Sulfo-NHS-LC-Biotin is water soluble,enabling reactions to be performed
组份
100mg/bottle
技术规格
| 分子量 | 556.59 |
| 间隔臂 | 22.4? |
| 纯度 | >95%on TLC |
我公司销售的Sulfo-NHS-LC-Biotin北京供应商,生物素化-epsilon-氨基己suān-N-羟基磺基丁二酰亚胺活化酯质量上佳,价格普惠,欢迎广大新老客户踊跃订购。
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文献和实验Detection of Cell Surface Dopamine Receptors
and enzyme-linked immunoabsorbent assay (ELISA) for detecting and quantifying levels of dopamine receptors expressed on the cell surface. In the biotinylation method, cell surface receptors are labeled with Sulfo-NHS-biotin. The charge on the sulfonyl
Detection of Tight Junction Barrier Function In Vivo by Biotin
molecules with different sizes have been widely used to determine the TJ barrier function. In the in vivo system, the tracer molecule Sulfo-NHS-Biotin was initially used in Xenopus embryo system and subsequently was successfully applied to a number of animal
下面的操作方法可以使约3~5分子的生物素与1分子的蛋白结合,对某些蛋白来说,生物素与蛋白的分子比可根据不同的生物素化要求进行调整。 1. 溶解2~10 mg 蛋白于1 ml 的BuphTm磷酸盐缓冲液中,并计算溶解的毫摩尔数。如果蛋白含有不恰当的缓冲液(如Tris或者甘氨酸),可以通过在PBS中透析或浓缩的方法除去。计算:蛋白质量(mg)/蛋白分子量(MW)=蛋白质的毫摩尔数。 2. 在打开之前,平衡生物素至室温。加2 mg Sulfo-NHS-Biotin于100 ul
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