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- 英文名:
Human Mesenchymal Stem Cells — Adult
- 细胞类型:
人正常原代细胞
- 物种来源:
人源
- 器官来源:
人源
- 运输方式:
干冰或液氮
- 年限:
液氮储存10年以上
- 生长状态:
冻存
- 规格:
500,000 cells/vial
Lifeline® Normal Human Mesenchymal Stem Cells-Adult (HMSC-Ad) provide an ideal culture model for the study of adult stem cell biology and differentiation processes.
HMSC-Ad are derived from adipose tissue and isolated from adult lipoaspirate. The cells have been plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure optimal phenotype and the highest viability and plating efficiency. 
Adult Mesenchymal Stem Cells can be expanded in an undifferentiated state for future differentiation to multiple lineages.
- Lifeline® Human Mesenchymal Stem Cells-Adult may be differentiated down the typical mesenchymal lineages, such as adipogenic, chondrogenic and osteogenic.
Mesenchymal Stem Cells-Adult are quality tested via flow cytometry to ensure the proper expression of multiple markers of mesenchymal stem cells. They are uniformly positive for CD29, CD44, CD73, CD90, CD105, and CD166. They are uniformly negative for CD14, CD31, CD34, and CD45.
Lifeline® has been a leader in crafting optimized media to expand Human Mesenchymal Stem Cell products in the undifferentiated state, as well as optimized media kits for inducing Adipogenesis, Chondrogenesis, and Osteogenesis. Additionally, Lifeline® provides convenient staining kits for staining the fully differentiated cells.
Mesenchymal Stem Cells -Adult are quality tested for:
- Morphology: Normal morphology for 3 passages
- Cell Viability: Minimum 70% viability when thawed from cryopreservation
- Sterility Testing: Negative for mycoplasma. Negative for bacterial and fungal growth
- Virus Testing: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
- Specific Staining: Positive* for CD29, CD44, CD73, CD90, CD105, CD166.
Negative* for CD14, CD31, CD34, CD45.
*Lifeline® defines positive expression as when greater than 95% of the cell population expresses that cell marker.
*Lifeline® defines negative expression as when less than 2% of the cell population expresses that cell marker.
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中国科学家团队合作发现促进多组织再生、延缓衰老的小分子代谢物
)以及年轻和衰老的间充质干细胞,系统揭示了一些跨物种保守的、再生相关的代谢通路。例如,再生能力强的生物样本更倾向于富集多胺代谢、尿嘧啶代谢和脂肪酸代谢通路。 进一步结合人类干细胞衰老的研究平台,研究人员对潜在的促再生代谢物进行了细致的筛选,发现小分子代谢物尿苷(Uridine)可以明显提升衰老人间充质干细胞的自我更新能力。 进一步研究表明,尿苷处理可以在 5 种小鼠的组织损伤模型(肌肉损伤模型、肝纤维化模型、毛发再生模型、心肌梗塞模型和关节炎模型)中助力损伤或病变组织的再生修复。在肌肉损伤模型中,尿苷
生长培养基(C-28010)培养剩余的一个样本作为阴性对照。5、间充质干细胞的分化培养至少培养3天,每48小时更换一次培养基。注意:诱导后只要1天就观察到细胞显著的形态学变化。6、收获和验证细胞MSC来源的神经细胞可以选择性的根据的“神经细胞标记物的检测”来进-步验证细胞的特征。 二、向成骨分化的操作流程 1、接种充质干细胞在24组织培养板中用MSC生长培养基接种间充质干细胞,每接种6x104个MSC(接种密度为3.15x104个/cm2)。2、间充质干细胞的生长要点:让细胞达到≥100
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