- ￥4920
- Adipogen
- AG-20B-0020-C100
- 瑞士
- 2026年02月28日
- EM, ICC, IHC, IP, WB
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
anti-Polyglutamylation Modification, mAb (GT335)
- 抗体英文名：
anti-Polyglutamylation Modification, mAb (GT335)
- 浓度：
1mg/ml
- 应用范围：
EM, ICC, IHC, IP, WB
- 供应商：
欣博盛生物
- 库存：
99
- 级别：
科研用
- 目录编号：
AG-20B-0020-C100
- 保质期：
见包装说明
- 克隆性：
单克隆
- 保存条件：
4°C
- 形态：
liquid
- 免疫原：
Octapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk).
- 规格：
100 ug
anti-Polyglutamylation Modification, mAb (GT335)产品由Adipogen公司生产，产地是瑞士。该产品货号为AG-20B-0020-C100，包装规格是100 ug。
如需购买该产品，询问折扣价/货期/技术问题，请联系Adipogen授权代理商——欣博盛生物
Product Details
Synonyms Polyglutamylated Tubulin; Glutamylated Tubulin; Postranslational Protein Glutamylation
Product Type Monoclonal Antibody
Properties
Clone GT335
Isotype Mouse IgG1κ
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Octapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk).
Application
Electron Microscopy (see Lit. 3)
Immunocytochemistry: (1:2000)
Immunohistochemistry: (paraffin sections; 1:1000)
Immunoprecipitation
Western Blot: (1:4000)
Optimal conditions must be determined individually for each application.
Crossreactivity All
Specificity
Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated α- and β-tubulin.
Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control
Mouse IgG1 Isotype Control
Other Product Data
Recognizes most forms of polyglutamylated tubulin (α- and β-tubulin), independent of the length of the glutamate side chains. No specificity to particular tubulin isoforms nor to tubulin from particular species are observed. Detects also other (poly)glutamylated proteins. Since no consensus modification site is known for protein (poly)glutamylation, the detection is not sequence-specific. However, an acidic environment of the modification site is required.
The use of the antibody at too high concentrations obscures its specificity in immunofluorescence.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
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文献和实验- Distribution of glutamylated alpha and beta-tubulin in mouse tissues using a specific monoclonal antibody, GT335: A. Wolff, et al.; Eur. J. Cell Biol. 59, 425 (1992)
- Polyglutamylation of nucleosome assembly proteins: C. Regnard, et al.; J. Biol. Chem. 275, 15969 (2000)
- Glutamylated tubulin: diversity of expression and distribution of isoforms: M.L. Kann, et al.; Cell Motil. Cytoskeleton 55, 14 (2003)
- Polyglutamylation Is a Post-translational Modification with a Broad Range of Substrates: J. van Dijk, et al.; J. Biol. Chem. 283, 3915 (2008)
- Unique post-translational modifications in specialized microtubule architecture: K. Ikegami & M. Setou; Cell Struct. Funct. 35, 15 (2010) (Review)
- Tubulin detyrosination promotes monolayer formation and api-cal trafficking in epithelial cells: S. Zink|et al.; J. Cell Sci. 125, 5998 (2012)
- The CP110-interacting proteins Talpid3 and Cep290 play overlapping and distinct roles in cilia assembly: T. Kobayashi, et al.; J. Cell Biol. 204, 215 (2014)
- CNS axons globally increase axonal transport after peripheral conditioning: F.M. Mar, et al.; J. Neurosci. 34, 5965 (2014)
- Microtubule detyrosination guides chromosomes during mitosis: M. Barisic, et al.; Science 348, 6236 (2015) (Supplement)
- SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture: M. Hilbert, et al.; Nat. Cell Biol. 18, 393 (2016)
- Loss of RPGR glutamylation underlies the pathogenic mechanism of retinal dystrophy caused by TTLL5 mutations: X. Sun, et al.; PNAS 113, E2925 (2016)
- Alterations in the balance of tubulin glycylation and glutamylation inphotoreceptors leads to retinal degeneration: M. Bosch Grau, et al.; J. Cell. Sci. 130, 938 (2017)
- The actin-MRTF-SRF transcriptional circuit controls tubulin acetylation via α-TAT1 gene expression: J. Fernández-Barrera, et al.; J. Cell Biol. 217, 929 (2018)
- iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications: J. Ou, et al.; Cell 173, 1 (2018)
- Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice: B. Ye, et al.; Nat. Commun. 9, 1261 (2018)
- Centrosome amplification arises before neoplasia and increases upon p53 loss in tumorigenesis: C.A.M. Lopes, et al.; J. Cell. Biol. 217, 2353 (2018)
- Differences in Intrinsic Tubulin Dynamic Properties Contribute to Spindle Length Control in Xenopus Species: W.G. Hirst, et al.; Curr. Biol. 30, 2184 (2020)
- ER proteins decipher the tubulin code to regulate organelle distribution: P. Zheng, et al.; Nature 601, 132 (2022)
- α- and β-tubulin C-terminal tails with distinct modifications are crucial for ciliary motility and assembly: T. Kubo, et al., J. Cell Sci. 136, 261070 (2023)
- Choroid plexuses carry nodal-like cilia that undergo axoneme regression from early adult stage: KH. Ho, et al.; Dev. Cell ahead of print (2023)
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
Adsroption of Anti-E. coli Antibodies Grow E. coli strain Y1090 in L broth at 37°C overnight. Transfer to 10 mM MgSO4 and store at 4°C for future use. These cells are good for about 1 week. Soak
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