- ￥4560
- Adipogen
- AG-20B-0012B-C100
- 瑞士
- 2026年02月28日
- ELISA
- Purified from concentrated hybridoma tissue culture supernatant.
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
anti-LAG-3 (human), mAb (17B4) (Biotin)
- 抗体英文名：
anti-LAG-3 (human), mAb (17B4) (Biotin)
- 靶点：
Recognizes human LAG-3.
- 浓度：
1mg/ml
- 应用范围：
ELISA
- 宿主：
Purified from concentrated hybridoma tissue culture supernatant.
- 供应商：
欣博盛生物
- 库存：
99
- 级别：
科研用
- 目录编号：
AG-20B-0012B-C100
- 保质期：
见包装说明
- 保存条件：
4°C
- 形态：
liquid
- 免疫原：
Synthetic peptide corresponding to 30 aa in the N-terminus of human LAG-3.
- 规格：
100 ug
anti-LAG-3 (human), mAb (17B4) (Biotin)产品由Adipogen公司生产，产地是瑞士。该产品货号为AG-20B-0012B-C100，包装规格是100 ug。如需购买该产品，询问折扣价/货期/技术问题，请联系Adipogen授权代理商——欣博盛生物
Synonyms Lymphocyte Activation Gene-3; FDC Protein; CD223
Product Type Monoclonal Antibody
Properties
Clone 17B4
Isotype Mouse IgG1κ
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Synthetic peptide corresponding to 30 aa in the N-terminus of human LAG-3.
Label/Conjugates Biotin
Application
ELISA: (detection)
Crossreactivity Human
Specificity
Recognizes human LAG-3.
Purity Detail Protein G-affinity purified.
Endotoxin Content <0.001EU/μg purified protein or <1EU /mg purified protein (LAL test; Lonza).
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control
Mouse IgG1 Isotype Control (Biotin)
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
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文献和实验- Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens: E. Baixeras, et al.; J. Exp. Med.176, 327 (1992)
- Cellular expression and tissue distribution of the human LAG-3-encoded protein, an MHC class II ligand: B. Huard, et al.; Immunogenetics 39, 213 (1994)
- Characterization of the major histocompatibility complex class II binding site on LAG-3 protein: B. Huard, et al.; PNAS 94, 5744 (1997)
- CD3/TCR complex-associated lymphocyte activation gene-3 molecules inhibit CD3/TCR signaling: S. Hannier, et al.; J. Immunol. 161, 4058 (1998)
- T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG-3): role of LAG-3/MHC class II interactions in cell-cell contacts: C.E. Demeure, et al.; Eur. J. Cancer 37, 1709 (2001)
- Immunological mechanisms elicited at the tumour site by lymphocyte activation gene-3 (LAG-3) versus IL-12: sharing a common Th1 anti-tumour immune pathway: E.D. Carlo, et al.; J. Pathol. 205, 82 (2005)
- The negative regulatory function of the lymphocyte-activation gene-3 co-receptor (CD223) on human T cells: L. Macon-Lemaitre and F. Triebel; Immunology 115,170 (2005)
- A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett. 235, 147 (2006)
- Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells: J. Bayry, et al.; J. Immunol. 178, 4184 (2007)
- Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation: C. Casati, et al.; J. Immunol. 180, 3782 (2008)
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
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