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- 文献和实验
- 技术资料
- 英文名：
VascuLife® VEGF Endothelial Medium Complete Kit
- 物种来源：
人
- 器官来源：
人源
- 运输方式：
冷藏或冷冻
- 年限：
1年
- 规格：
500ml
VascuLife® VEGF (containing Vascular Endothelial Growth Factor) is a low serum medium optimized for the rapid proliferation of Human Endothelial Cells, including:
- Human Umbilical Vein Endothelial Cells (HUVEC)
- Aortic Endothelial Cells
- Other human large-vessel endothelial cells
VascuLife® culture medium supports the growth of these cells in a low serum environment.
VascuLife® Basal Medium contains no antimicrobials and no phenol red.
VascuLife® VEGF Medium contains rh VEGF, which may affect stimulation assays. Lifeline® recommends using VascuLife® EnGS for these types of studies.
- Kit Format: VascuLife® culture medium is offered in a kit format composed of basal medium and associated supplements and growth factors called ” LifeFactors® .” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
- Protective Packaging: VascuLife® Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors® (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.
LifeFactors® include:
ISO-Style Quality Testing for Guaranteed Consistency and Reproducible Results
VascuLife® VEGF medium grows HUVEC and other human large-vessel endothelial cells at rates that meet or exceed other commercially available serum-containing media while maintaining excellent cell morphology. In comparisons with other commercially-available media, Vasculife® VEGF shows superior proliferation at different seeding densities.
Shown at left: HUVEC, passage 2, 4 days after inoculation with 2,000 cells/cm2 (100X).
Shown below: HUVEC inoculated at low seeding density in 24-well plates. Higher fluorescence represents a greater number of cells per well and therefore better proliferation.
Every production lot of VascuLife® VEGF Endothelial Cell Culture Medium is extensively tested using Endothelial Cells.
- Sterility Testing: Negative for bacteria and fungal growth
- pH: 7.8 +/- 0.3
- Cell Testing: Rate of proliferation and morphology
- Osmolality: 270 +/- 10 mOsm
- Endotoxin: < 0.5 EU/mL
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文献和实验Vybrant® DyeCycle Green and Orange Stains
DyeCycle™ Staining Protocol This basic protocol is optimized using Jurkat cells suspended in complete medium (RPMI/10% fetal bovine serum) and stained with Vybrant® DyeCycle™ stain at 37?C. Remove the Vybrant® DyeCycle™ Green stain
上新：细胞治疗原料扩容:GMP级重组人VEGF165 - 血管再生核心因子
使用，在特定的无血清培养基中，专门用于诱导多能干细胞向中胚层（Mesoderm）和造血祖细胞（Hematopoietic progenitor cells）分化。 图2.人 iPSC 向红细胞的分化[2] 02 血管生成与内皮细胞培养 VEGF165是诱导多能干细胞（iPSC）、胚胎干细胞（ESC）向血管内皮细胞定向分化的关键特异性诱导因子。 被广泛应用于原代内皮细胞培养,如人脐静脉内皮细胞（HUVEC）的培养、存活及增殖
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
medium and discard. To the bacterial pellet add 250 u Solution I/RNase A. Resuspend cells completely by vortexing or pipetting up and down. Complete resuspension of cell pellet is vital for obtaining good yields. 4. Add 250 u Solution II and mix
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