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- 2025年07月09日
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文献和实验Sauer:Lysing E. coli with Lysozymes
. Hen Egg White Lysozyme I prepare the lysozyme fresh each time from lyophilized powder. Pre-warm the bottle to prevent moisture from condensing in the bottle when it''s opened. Pure B-Per and most lysis buffers don''t seem to allow
to by making antibody, can frequently be one unique structure on the antigen. In hen egg white lysozyme, a glutamine at position 121 (Gln 121) protrudes away from the antigen surface. In this view, Gln 121 is circled. The antibody is not shown. The following
,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
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