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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
参照说明书
- 英文名:
AptaFluor SAH Methyltransferase Assay (10,000 Assay, 384 Well)
- 库存:
1
- 供应商:
北京博蕾德生物科技有限公司
- 规格:
384 Well
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文献和实验min,The assay can be interrupted at this point,and the samples kept at -20℃ until ready to proceed. Vortex 5-10s Spin in cold room microfuge for 15 min. Pour off supernatant. Add 500 ml 70% ETOH.Vortex. Spin
, dilute 30 ml aliquots by adding LB. For cultured cells, I have been diluting to 0.5 mg protein per 15 ml LB. For frozen tissues, I have been diluting to 5 mg protein per 15ml. This assay is linear up to 4 mg protein from cultured cells and 10 mg protein
100% ETOH. Store at �70 ° C for 1 hour and centrifuge 30 min. at >10,000 g. Pour the ETOH out, air dry the pellets. Resuspend in 10-20 m l TE and determine DNA concentration using a spectro-photometer. 3.1.2 Ligation
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