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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
参照说明书
- 英文名：
AptaFluor SAH Methyltransferase Assay (1,000 Assay, 384 Well)
- 库存：
1
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
384 Well
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文献和实验has made some useful shortcuts that work well if you are careful.Here is .This assay can be used to measure activity in small,snap frozen pieces of tissue (e.g.,colon polyps),or in cultured cells. 1.Homogenize Cells/Tissues For cells in culture: Harvest
Quantitate proteins in 15 ml aliquots of lysates (in duplicate) by using a Bradford assay (I have been using the Biorad kit), using BSA as standard. Don't forget to add 15 ml LB to each BSA sample and to the blank tube. Use the same LB batch as that used
100% ETOH. Store at �70 ° C for 1 hour and centrifuge 30 min. at >10,000 g. Pour the ETOH out, air dry the pellets. Resuspend in 10-20 m l TE and determine DNA concentration using a spectro-photometer. 3.1.2 Ligation
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