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- 详细信息
- 文献和实验
- 技术资料
- 库存:
53
- 英文名:
dNTP/dUTP Mixture
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
阴凉干燥
- 规格:
1 ml
特别提示:包括dNTP/dUTP混合物在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:dNTP/dUTP混合物
英文名称:dNTP/dUTP Mixture
产品货号:MT0112
产品规格:1 ml
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文献和实验. expressed in EACH dNTP). THEN dilute this (or aliquot) 1/10 with WATER - aliquot into 100 ul amounts and freeze. ii) Prepare a 1 mM stock of dNTPs with dTTP substituted to 10% (w/w) by digoxigenin-11-dUTP (DIG-dUTP) for use as a labelling mix for PCR
Calculating Concentrations for PCR
supplied (by Boehringer Mannheim) at 25 nmol/25ul = 1 umol/ml = 1mM; final concentration of DIG-dUTP must be 1/10th that of other nucleotides, and [DIG-dUTP] + [dTTP] must = [any other dNTP]. Therefore to get a 1 mM dNTP stock one must dilute DIG-dUTP stock
Calculating Concentrations for PCR
(by Boehringer Mannheim) at 25 nmol/25ul = 1 umol/ml = 1mM; final concentration of DIG-dUTP must be 1/10th that of other nucleotides, and [DIG-dUTP] + [dTTP] must = [any other dNTP]. Therefore to get a 1 mM dNTP stock one must dilute DIG-dUTP stock 1/10.
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