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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C储存
- 英文名:
dNTP Mix
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
D292587-1ml
英文名:dNTP Mix
纯度或规格:25mM each
SPU:D292587
CAS:-
存储温度:-20°C储存
运输条件:超低温运输
查看阿拉丁官网此产品相关对应页面:
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文献和实验2 concentration is 1-4mM, under the standard reaction conditions specified. In our experiments, at a final dNTP concentration of 0.2mM, a MgCl2 concentration ranges of 1.5±0.25mM (in traditional PCR buffer) and of 2.0±0.5mM (in PCR buffer with (NH4)2SO4
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
100 mM dNTP Set PCR grade (Life Technologies; Cat # 10297-018) Primers: Poly d(T) [20-mers] 2.5 mg/mL (avoids labeling the gDNA and rRNA.) Anchored poly d(T) primers (Archaic) Random Hexamer primers (3mg/mL) (Life
Troubleshooting for PCR and multiplex PCR
of these ingredients in the PCR mix are: 50 mM KCl; 10 mM Tris-HCl; 1.5 mM MgCl2).QUESTIONS and SOLUTIONS1. I get (many) longer unspecific products. What can I do? Decrease annealing time Increase annealing temperature Decrease extension time Decrease extension
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