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文献和实验Hydrogen Peroxide Assay for Amine Oxidase Activity
Amine oxidase activity can be assessed by measuring the conversion of substrate to product, or by measuring the formation of concomitantly formed hydrogen peroxide. Fluorometry offers greater sensitivity and selectivity than conventional
Synthesis of O-Glycopeptides and Construction of Glycopeptide Microarrays
O -Glycosylation of proteins is an important modification which affects biological function and immunity. In this chapter, we provide protocols for efficient solid-phase O -glycopeptide synthesis (SPGPS) and protocols for the construction
An Isotope Coding Strategy for Proteomics Involving Both Amine and Carboxyl Group Labeling
of amine and carboxyl groups generated during proteolysis as a means of coding. Carboxyl groups produced during proteolysis incorporate 18 O from H2 18 O. Peptides from the C-terminus of proteins were not labeled with 18 O
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