- ¥1580 - 2480
- gelatins
- jlcR12247
- 国内
- 2025年07月06日
- WB,ELISA等
- 人/动物/植物
10 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
64
- 克隆性：
单克隆
- 保质期：
1年
- 抗体英文名：
NAC1
- 抗体名：
转录因子NAC1抗体
- 适应物种：
人/动物/植物
- 应用范围：
WB,ELISA等
- 浓度：
1mg/ml
- 保存条件：
-20 °
- 规格：
100ul/200ul
| 规格： | 100ul | 产品价格： | ¥1580.0 |
|---|---|---|---|
| 规格： | 200ul | 产品价格： | ¥2480.0 |
产品货号 : jlcR12247
英文名称 : NAC1
中文名称 : 转录因子NAC1抗体
别 名 : BTBD14B; BEND8; BTB Domain Containing 14B; BTB/POZ domain-containing protein 14B; btbd14b; btbd14b; FLJ37383; NAC-1; NAC1; Nacc1; NACC1_HUMAN; Nucleus accumbens-associated protein 1.
研究领域 : 肿瘤 发育生物学 干细胞 转录调节因子
抗体来源 : Rabbit
克隆类型 : Polyclonal
交叉反应 : Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
产品应用 : WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 : 57kDa
细胞定位 : 细胞核 细胞浆
性 状 : Lyophilized or Liquid
浓 度 : 1mg/ml
免 疫 原 : KLH conjugated synthetic peptide derived from human NAC1/BTBD14B:311-400/527
亚 型 : IgG
纯化方法 : affinity purified by Protein A
储 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 : NAC1 is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. BTBD14B (BTB/POZ domain-containing protein 14B), also known as NACC1 (nucleus accumbens associated 1), BEND8 or NAC1, is a 527 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one BTB (POZ) domain. Existing as a homooligomer that interacts with HDAC3 and HDAC4, BTBD14B functions as a transcriptional repressor that influences the transcriptional activity of CRIF1 and is required for proteasome recruitment to the nucleus and cytoplasm in dendritic spines. BTBD14B is overexpressed in multiple carcinomas, suggesting a role in tumor development and metastasis.
Function:
Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
Subunit:
Homooligomer; mediated by the BTB domain. Interacts with HDAC3 and HDAC4. Interacts (via BTB domain) with CUL3, PSMD7 AND RCOR1.
Subcellular Location:
Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.
Tissue Specificity:
Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
DISEASE:
Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
Similarity:
Contains 1 BEN domain.
Contains 1 BTB (POZ) domain.
SWISS:
Q96RE7
Gene ID:
112939
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
英文名称 : NAC1
中文名称 : 转录因子NAC1抗体
别 名 : BTBD14B; BEND8; BTB Domain Containing 14B; BTB/POZ domain-containing protein 14B; btbd14b; btbd14b; FLJ37383; NAC-1; NAC1; Nacc1; NACC1_HUMAN; Nucleus accumbens-associated protein 1.
研究领域 : 肿瘤 发育生物学 干细胞 转录调节因子
抗体来源 : Rabbit
克隆类型 : Polyclonal
交叉反应 : Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
产品应用 : WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 : 57kDa
细胞定位 : 细胞核 细胞浆
性 状 : Lyophilized or Liquid
浓 度 : 1mg/ml
免 疫 原 : KLH conjugated synthetic peptide derived from human NAC1/BTBD14B:311-400/527
亚 型 : IgG
纯化方法 : affinity purified by Protein A
储 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 : NAC1 is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. BTBD14B (BTB/POZ domain-containing protein 14B), also known as NACC1 (nucleus accumbens associated 1), BEND8 or NAC1, is a 527 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one BTB (POZ) domain. Existing as a homooligomer that interacts with HDAC3 and HDAC4, BTBD14B functions as a transcriptional repressor that influences the transcriptional activity of CRIF1 and is required for proteasome recruitment to the nucleus and cytoplasm in dendritic spines. BTBD14B is overexpressed in multiple carcinomas, suggesting a role in tumor development and metastasis.
Function:
Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
Subunit:
Homooligomer; mediated by the BTB domain. Interacts with HDAC3 and HDAC4. Interacts (via BTB domain) with CUL3, PSMD7 AND RCOR1.
Subcellular Location:
Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.
Tissue Specificity:
Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
DISEASE:
Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
Similarity:
Contains 1 BEN domain.
Contains 1 BTB (POZ) domain.
SWISS:
Q96RE7
Gene ID:
112939
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
基因表达调控的重点。现在的基因数据库信息丰富，拿到基因及其启动子序列非常简单。那么问题又来了，拿到启动子序列以后，下一步怎么找相关的调控蛋白/转录因子呢？生物信息学方法预测？你会得到很多可能的目标调控蛋白/转录因子，还要做实验一个一个验证。凝胶迁移（EMSA），染色质免疫共沉淀（ChIP）？只能针对已知能与启动子结合的调控蛋白/转录因子，而且还需要相应探针/抗体，对于大量筛选无能为力。美国Signosis的转录因子(结合启动子)微孔板芯片检测试剂可以方便、高效地解决这一问题。该方法专门用于筛查与特定DNA序列
原理： 将染色质和与之相互作用的转录因子和组蛋白通过甲醛等物质交联起来，然后通过超声将染色质打碎成小片段，加入针对特定转录因子或特殊修饰的组蛋白抗体，通过 Protein A/Protein G 微球或磁珠将抗体-转录因子-染色质复合物拖下来，通过 PCR 或测序的方法检测与目的蛋白相结合的 DNA 序列，进而研究这些转录因子在细胞发育或者生长中的作用位点。 ✦ ChIP-seq： ChIP-seq 将 ChIP 技术与二代测序相结合，将 ChIP 下来的 DNA 进行二代测序文库构建，能够获取
电极移动距离的大小是同其分子量的对数成反比。如果某种 DNA 分子结合上一种特殊的蛋白质，那么由于分子量的加大它在凝胶中的迁移作用便会受到阻滞，于是朝正极移动的距离也就相应的缩短，因而在凝胶中出现滞后的条带，这就是凝胶阻滞实验的基本原理。 3. 过程： 首先制备细胞蛋白质提取物（理论上其中含有某种特殊的转录因子） 用放射性同位素标记待检测的 DNA 片段（含有转录因子的结合位点） 这种被标记的探针 DNA 同细胞蛋白质提取物一起进行温育，于是产生DNA -蛋白质复合物，在控制使 DNA -蛋白质保持结合状态
技术资料暂无技术资料 索取技术资料
转录因子NAC1抗体
¥1580 - 2480
