- ¥1572 - 3512
- Thermo Scientific™
- K0491
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Thermo Scientific™ GeneJET Plasmid Maxiprep Kit
- 供应商：
上海辅泽商贸有限公司
- 规格：
K0491-10 preps/K0492- 25 preps
| 规格： | K0491-10 preps | 产品价格： | ¥1572.0 |
|---|---|---|---|
| 规格： | K0492- 25 preps | 产品价格： | ¥3512.0 |
货号： K0491
相关应用：联系我们获取支持 ›
| 货号 | 单位规格 | 单价 (CNY) | 数量 | |
|---|---|---|---|---|
| K0491 | 10 preps | 1,572.00 | ||
| K0492 | 25 preps | 3,512.00 |
规格
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
|---|---|
| Sample Type (General): | Bacteria |
| Downstream Application: | Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Transfection, Transformation |
| Product Line: | GeneJET |
| Product Size: | 10 preps |
描述
Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 10 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.Highlights
• Efficient—high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
• High concentration of DNA purified—> 400 ng/ µL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns
Applications
• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• Transfection of robust cell lines
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription
For Research Use Only. Not for use in diagnostic procedures.
图表
High yields of plasmid purified with GeneJET Maxiprep Kit
Efficient transfection of robust cell lines with plasmid DNA purified using GeneJET Maxiprep Kit
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文献和实验Maxiprep preparation of Plasmid DNA
实验概要 The PureLink™ HiPure Plasmid DNA Maxiprep Kit allows purification of 500–850 μg of high-quality plasmid DNA from 100–200 mL overnight E. coli cultures in ~2 hours when cloning high copy number plasmids.
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Pierce Chromatin Prep Module Thermo 1 kit 17-295 ChIP Assay Kit millipore 1 kit 17-371 EZ-ChIP™ Kit
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