M2 培养基

性质
Related Categories    Core Bioreagents, Life Science Reagents for RNAi, Media / Serum, Mouse Embryo Culture, Mouse Embryo Culture Media,
Research Essentials, 特殊培养基系统, 细胞培养
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Quality Level      GMP
sterility      sterile-filtered
form      liquid
impurities      endotoxin, tested
suitability      suitable for mouse embryo
storage temp.      2-8°C
产品描述
Application
Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.

Quality
溶液中的沉淀不影响本品性能。

Reconstitution
添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。

Analysis Note
This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.