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文献和实验The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers
concentration in the culture medium by using Morin (a fluorescent histochemical indicator for Al). Because Al affects different processes involved in the signal transduction pathway such as phospholipase C (PLC) activity, which could serve as selection marker
, and 20 in this volume). Using the naturally occurring leader sequence, recombinant enzymes are secreted by insect cells and are easy to purify in a one-step procedure when insect cells are grown in a serum-free medium.
Replication timing by density transfer
C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered
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