One-Step Purification and Biochemical Characterization of Recombinant Pancreatic Lipases Expressed in Insect Cells
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The baculovirus expression system is very convenient to produce recombinant pancreatic lipases and mutants thereof, in substantial amounts (10–50 mg of enzyme per liter of culture) for structure-function studies (see also Chapters 17 , 18 , and 20 in this volume). Using the naturally occurring leader sequence, recombinant enzymes are secreted by insect cells and are easy to purify in a one-step procedure when insect cells are grown in a serum-free medium.
