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文献和实验Human Placental Alkaline Phosphatase Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike other AP proteins typically expressed by cells. A few cell types (HeLa cells, for example
Look Ma, No Archenteron! Sulfate role in sea urchin early development
of the extracellular matrix within the blastocoel of a developing sea urchin (1974). Developing sea urchin embryos were raised in either artificial sea water or sulfate-free sea water, fixed, and stained for alkaline phosphotase enzyme activity
CLONING FROM BEGINNING TO END 详细的克隆操作过程【UCSF】
,resuspend with dW d.Add Alkaline Phosphatase with buffer (see below).1μg DNA requires a 10μl reaction (scale up as desired)... Reaction should end up: 1 unit Alkaline Phosphatase(AP or "CIP")/ 1μg DNA Dilute 10X buffer to 1X Bring up to volume with dW
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