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文献和实验Methylene Blue DNA staining protocol
Methylene Blue DNA staining protocol Protocol: Load 2-5X the amount of DNA that would give bands of moderate intensity on an ethidium bromide stained gel. Typically this is something on the order of 0.5-2.5 µg
Photochemical crosslinking is an approach that is widely used to detect and analyze interactions between proteins and nucleic acids. The approach involves illuminating a mixture of protein and nucleic acid with light of a suitable wavelength
Hematoxylin and Eosin (HE) Staining
1) (changes the stain from purple to blue) Running H2 O 5 min 80% EtOH x1 Eosin 15" 95% EtOH x2 100% EtOH Xylene 3 min x2Mounting Wipe off the xylene off the back of a slide on a paper towel Using a clean
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