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产品名称：Recombinant Human PTH 1-84/Parathyroid hormone/Parathormone
Known as Parathyroid Hormone; PTH; Parathormone; Parathyrin
Derived from E.coli
Purity Greater than 95% as determined by reducing SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150mM NaCl, 5% Mannitol, pH 4.0.
Shipping The product is shipped at ambient temperature.
Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Endotoxin Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Background Parathyroid hormone is the most important endocrine regulator of calcium and phosphorus concentration in extracellular fluid. This hormone is secreted from cells of the parathyroid glands and finds its major target cells in bone and kidney. Another hormone, parathyroid hormone-related protein, binds to the same receptor as parathyroid hormone and has major effects on development. Like most other protein hormones, parathyroid hormone is synthesized as a preprohormone. After intracellular processing, the mature hormone is packaged within the Golgi into secretory vesicles, the secreted into blood by exocytosis. Parathyroid hormone is secreted as a linear protein of 84 amino acids.
Recombinant Human Parathyroid Hormone is produced by our E.coli expression system and the target gene encoding Ser32-Gln115 is expressed.
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文献和实验【分享】Human press METHODS IN MOLECULAR BIOLOGY
by Minteer, Shelley D. 2005 322.Xenopus Protocols Cell Biology and Signal Transduction. Edited by Liu, X. Johné 2005 323.Arabidopsis Protocols 2 nd. Edited by Salinas, Julio 2005 324.Hormone Assays in Biological Fluids Edited by Wheeler, Michael J. 2005
Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
feedback occurs when the level of one substance regulates secretion of a hormone. For example, a low serum calcium level stimulates the parathyroid glands to secrete parathyroid hormone (PTH), and a high serum calcium level inhibits PTH secretion
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