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文献和实验Reaction Mix: It is again convenient to make up a master mix to be aliquotted out to amplify all samples, if they are to use the same primers. If not, modify master mix by simply leaving out primers. Total volume: 50ul / rxn x N + 5-10
and make fresh with each use. Calculation for 1.25 Units/ul stock: (1.25 Units/ul) X (1 ul) = (.025 Units/ul) X (50 ul) To make the master mix for one reaction add: * 5 ul 10X buffer * 4ul Each nucleotide (1ul each of dATP, dCTP, dGTP, dTTP)) * 20 ul
START" PCR.NOTE:USE PLUGGED PIPETTE TIPS: prevents aerosol contamination of pipettes.Use of detergents is recommended only for Taq from Promega (up to 0.1% v/v, Triton X-100 or Tween-20). DMSO apparently allows better denaturation of longer target
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