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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb
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western blot,免疫沉淀(IP),流式细胞(Flow Cyt)
小鼠
大量
人,小鼠,大鼠,仓鼠,驴,其它,其他,斑马鱼,细菌,猪
详见MSDS文件
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详见说明书
CST
1
-20°c
200 ul (40 western blots)/600 ul (120 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
规格: | 产品价格: | ¥请询价 | |
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规格: | 200 ul (40 western blots) | 产品价格: | ¥请询价 |
规格: | 600 ul (120 western blots) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
Have you tried your application using our XP® monoclonal antibodies? Try product: 4370
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
---|---|---|---|---|
W IP F | H M R Hm Mk Mi Dm Z B Pg | Endogenous | 42, 44 | Mouse IgG1 |
Applications Key: W=Western Blotting IP=Immunoprecipitation F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey Mi=Mink Dm=D. melanogaster Z=Zebrafish B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Tyr204. This antibody does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. This antibody may cross-react with an unknown cytoskeletal protein in some cell lines as visualized by immunofluorescence.
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase.
Specificity and sensitivity of the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb: This antibody reacts specifically with as little as 50 pg of phosphorylated MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated MAP kinase by Western blotting.
Western blot analysis of extracts from FGF treated SK-N-MC cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (upper) or control p44/42 MAPK (Erk1/2) Antibody #9102 (lower).
Flow cytometric analysis of Jurkat cells, untreated (red) or PMA-treated (blue) ; and untreated (green) or PMA-treated (purple) following 90-minute CIP treatment, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb.
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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