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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Mouse mAb
- 抗体英文名:
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Mouse mAb
- 靶点:
详见说明书
- 浓度:
1.0mg/ml
- 应用范围:
WB
- 宿主:
Mouse
- 供应商:
上海钰博生物科技有限公司
- 库存:
大量
- 目录编号:
3355
- 抗原来源:
Mouse
- 保质期:
1年
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20°C
- 形态:
液体或冻干粉
- 亚型:
详见说明书
- 免疫原:
详见说明书
- 规格:
50μg/50μl, 100μg/100μl
-
- Catalog No. : Yb--3355
- Size : 50μg/50μl, 100μg/100μl
-
- Mol Weight : 44/42KDa
- UniProt :
-
- Isotype : Mouse IgG1
- Clonality : Monoclonal
-
- Price : $265/100μg
- Availability : 3-4 days
Category
- Primary Antibodies
- Secondary antibody
- Loading control / tag antibody
Details
Alternative Names:
MAPK3; MK03; MNK1; p44-ERK1; P44-ER; ERK-1; ERK1; ERT2; kinase ERK1; MAP kinase 1; MAPK 1
Application:
WB
Reactivity:
Human,Mouse
Dilution:
WB 1:500
![]() |
Western blot analysis of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) from EGF-treated A549 cells and PDGF-treated NIH/3T3 cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Mouse mAb (201245,1:500 diluted,upper) and p44/42 MAPK (Erk1/2) Mouse mAb |
Purification:
Affinity-chromatography
Specificity:
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Mouse mAb detects endogenous levels of total Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
Immunogen:
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) synthetic peptide conjugated to KLH.
Description:
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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文献和实验:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
) is accompanied with or inactivates the GSK activity. However, it is still not conclusive what the role of Tyr phosphorylation (Y216 fro beta, Y279 for alpha) on GSK activity is. One reference is a review paper published in Cell in 2001, you may take a quick
secondary antibody were purchased from GIBCO-BRL. Phosphospecific rabbit polyclonal antibodies against 180Thr and 182Tyr dual-phosphorylated p38, against total p38 (phosphorylation state independent), against 204Tyr-phosphorylated ERK1/2 and against total
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