- 询价
- Cell Signaling Technology
- 3407
- USA
- 2026年01月09日
- W, IP
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
IRS-1 (D23G12) Rabbit mAb
- 抗原：
synthetic peptide corresponding to the sequence surrounding Ser270 of human IRS-1
- 应用范围：
W, IP
- 适应物种：
H,M,R,Mk
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (40 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (40 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 180 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | IRS-1 (D23G12) Rabbit mAb detects endogenous levels of total IRS-1 protein. The antibody does not cross-react with IRS-2. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Ser270 of human IRS-1. Western Blotting
Western blot analysis of MCF7, C2C12 and RD cell lines using IRS-1 (D23G12) Rabbit mAb. |
| Background | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验#:RT1510, HuaAn biotechnology , inc.4.IRS1, Mouse mAb IgG1, Cat#:M1210-2. HuaAn biotechnology , inc.5.XBP1, Rabbit polyclonal antibodies, SC-7160, Santa Cruz biotechnology,inc.6.b-Actin, Mouse monoclonal IgG1, SC-47778. Santa Cruz biotechnology
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
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