Cdc6 (C42F7) Rabbit mAb

3387:

Western Blotting

Cdc6 (C42F7) Rabbit mAb detects endogenous levels of total cdc6 protein.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human cdc6.

Western Blotting

Western blot analysis of extracts from various cell types using Cdc6 (C42F7) Rabbit mAb.

The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle. Origins of replication are dispersed throughout the genome, and their activities are regulated via the sequential binding of pre-replication and replication factors. The origin recognition complex (ORC) is thought to be bound to chromatin throughout the cell cycle (1,2). The pre-replication complex (Pre-RC) forms in late mitosis/early G1 phase beginning with the binding of CDT1 and cdc6 to the origin, which allows binding of the heterohexameric MCM2-7 complex. The MCM complex is thought to be the replicative helicase, and formation of the pre-RC is referred to as chromatin licensing. Subsequent initiation of DNA replication requires the activation of the S-phase promoting kinases cdk2 and cdc7. Cdc7, which is active only in complex with its regulatory subunit dbf4, phosphorylates MCM proteins bound to chromatin and allows binding of the replication factor cdc45 and DNA polymerase (3,4).Replication licensing is controlled in part by the degradation of cdc6 in quiescent cells. Phosphorylation of cdc6 by cdk2 prevents its degradation, allowing pre-replication complexes to form (5). Cdc6 has recently been shown to play an important role in the intra-S-phase p21 Waf1/Cip1-dependent DNA damage response (6,7). Both cdc6 and CDT1 are degraded by the ubiquitin proteasome pathway in response to DNA damage associated with re-replication (8).

1. Okuno, Y. et al. (2001) EMBO J 20, 4263-77.
2. McNairn, A.J. et al. (2005) Exp Cell Res 308, 345-56.
3. Bell, S.P. and Dutta, A. (2002) Annu Rev Biochem 71, 333-74.
4. Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
5. Mailand, N. and Diffley, J.F. (2005) Cell 122, 915-26.
6. Kan, Q. et al. (2008) J Biol Chem 283, 17864-72.
7. Kan, Q. et al. (2008) Proc Natl Acad Sci USA 105, 4757-62.
8. Hall, J.R. et al. (2008) J Biol Chem 283, 25356-63.

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For Research Use Only. Not For Use In Diagnostic Procedures.