- 询价
- Cell Signaling Technology
- 5059
- USA
- 2025年10月03日
- 免疫荧光（IF）
- 兔
- 人,小鼠,大鼠,驴,其他,细菌,狗
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
α-Tubulin (11H10) Rabbit mAb (Alexa Fluor 555 Conjugate)
- 抗原：
/
- 应用范围：
免疫荧光（IF）
- 宿主：
兔
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 适应物种：
人,小鼠,大鼠,驴,其他,细菌,狗
- 抗原来源：
/
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Cytoskeletal Signaling
α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate) #5059
PhosphoSitePlus ® protein, site, and accession data: TUBA1B
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC | H M R Mk Dm B (Dg) | Endogenous | Rabbit IgG |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster B=Bovine Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5059:
- Immunofluorescence
Specificity / Sensitivity
α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total α-tubulin protein. This antibody does not cross-react with recombinant β-tubulin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human α-tubulin. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluroescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (11H10) Rabbit mAb #2125.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
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家兔（Rabbit,Oryctolagus curiculus）
家兔（Rabbit,Oryctolagus curiculus） 一、生物学特性和解剖生理特点 1．家兔属于哺乳纲、啮齿目、兔科、草食性哺乳动性。 2．家兔体小力弱、胆小怕惊、怕热、怕潮，喜欢安静、清洁、干燥、凉爽的环境，不能忍受污秽的条件。 3．是食草类（Herbivorous）单胃动物。饲养原则是以青粗食米为主，精饲料为辅。喜欢独居，白天活动少，都处于假眠或休息状态，夜间活动大，吃食多。有啃木、扒土的习惯
'）2 Fragment（Alexa Fluor® 555 Conjugate）#4413 Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 647 Conjugate）#4414 抗小鼠： Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4408 Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor
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