- 询价
- Cell Signaling Technology
- 3981
- USA
- 2026年01月02日
- IP
- Rabbit
- M,H,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Gα(o) Antibody (IP Preferred)
- 抗原：
synthetic peptide corresponding to residues surrounding Arg15 of human Gα(o)
- 应用范围：
IP
- 宿主：
Rabbit
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 适应物种：
M,H,R
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| IP | M (H) (R) | Endogenous | 40 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Gα(o) Antibody (IP Preferred) detects endogenous levels of total Gα(o) protein and is recommended for immunoprecipitation. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg15 of human Gα(o). Antibodies are purified by protein A and peptide affinity chromatography. IP
Immunoprecipitation and western blot analysis of extracts from mouse brain. Lane 1 contains lysate input, lane 2 was immunoprecipitated with Gα(o) Antibody (IP Preferred) and lane 3 was immunoprecipitated with non-specific rabbit IgG. Western blot analysis was performed using Gα(o) Antibody #3975. |
| Background | Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3). Gα(o) is the most abundant G protein in the brain and couples to serotonin, dopamine, GABA (B), opioid, glutamate and cholinergic receptors (4). Gα(o) -/- mice have neurological defects such as tremors, seizures and poor motor coordination (5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验，该对照组称为output 组。 内源性 co-IP 实验，如果最终结果为阳性，则可以证明两个蛋白之间存在相互作用；但是结果为阴性，是否两个蛋白就一定不发生相互作用了了呢？也不一定。有可能是两个蛋白是弱相互作用力，这时候可能需要交联剂协助，锁定强化弱互作蛋白；也有可能是内源蛋白表达量低，可先做过表达 co-IP 作为对照。 实验前的思考 1、抗体----选经过 IP 验证的抗体，减少假阳性概率 2、抗体结合蛋白---Protein A, Protein G, Protein A/G, Protein
IP Issues in the Therapeutic Antibody Industry
The following section is related to IP issues in the therapeutic Antibody industry. A rough overview over protected enabling techniques and compounds is provided, in order to facilitate the entry into freedom to operate studies. Furthermore
C18 H24 O3 ，雌甾 -1， 3， 5（ 10） -三烯 -3， 16α， 17β -三醇。是一种性激素，可从妊妇尿中检出。雌激素的作用比雌酮弱。在胎儿的肾上腺里生成的脱氢表雄酮，在肝脏里经 16α羟基化后，在胎盘里芳构化而成为雌三醇。随着妊娠的进行，在尿中的排出量逐渐增加，至分娩后锐减。作为缓和的性激素试剂，经口服可治疗更年期障碍等。
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