Applications Key: W=Western Blotting Reactivity Key: H=Human M=Mouse R=Rat Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Notch1 (C44H11) Rabbit mAb detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~ 300 kDa) and the transmembrane/intracellular region NTM (~120 kDa).
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro2439 of human Notch1.
Western Blotting
Western blot analysis of total extract from HBP-ALL cells using Notch1 (C44H11) Rabbit mAb. The full-length (FL) and transmembrane/intracellular region (NTM) are indicated.
Western Blotting
Western blot analysis of extracts from various cell lines using Notch1 (C44H11) Rabbit mAb. The transmembrane/intracellular region (NTM) is indicated.
Background
Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4).