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- Cell Signaling Technology
- 3268
- USA
- 2026年01月02日
- W
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Notch1 (C44H11) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Pro2439 of human Notch1
- 应用范围：
W
- 供应商：
CST
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
H,M,R
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H (M) (R) | Endogenous | 120, 300 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Notch1 (C44H11) Rabbit mAb detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~ 300 kDa) and the transmembrane/intracellular region NTM (~120 kDa). |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro2439 of human Notch1. Western Blotting
Western blot analysis of total extract from HBP-ALL cells using Notch1 (C44H11) Rabbit mAb. The full-length (FL) and transmembrane/intracellular region (NTM) are indicated. Western Blotting
Western blot analysis of extracts from various cell lines using Notch1 (C44H11) Rabbit mAb. The transmembrane/intracellular region (NTM) is indicated. |
| Background | Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
qp。在 CHO-MAb 分批补料培养的情况下，使用 5 倍浓缩氨基酸进料（``Hi'' 渗透法）会导致培养物的渗透压逐渐从 320 mOsm / kg 升高到约 420 mOsm / kg（图 2）与保持低渗透压（降至 280 mOsm / kg）的 1x 补料添加培养物（'Lo' 渗透状态）相比。图 1. 渗透压对批次 MAb-CHO 培养的影响。（A）C44 和（B）C56 细胞系在不同分批培养渗透压下的活细胞浓度（VCC）与时间的关系。（C）qp 和（D）C44 和 C56 的最终 MAb
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
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