- 询价
- Cell Signaling Technology
- 3969
- USA
- 2026年05月28日
- 免疫荧光（IF）
- 兔
- ,
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- 文献和实验
- 技术资料
- 抗体英文名：
Rabbit (DA1E) mAb IgG XP Isotype Control (Alexa Fluor 555 Conjugate)
- 抗原：
/
- 应用范围：
免疫荧光（IF）
- 宿主：
兔
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 抗原来源：
/
- 保质期：
详见说明书
- 适应物种：
,
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul /<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Complementary Reagents
Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 555 Conjugate) #3969
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC | Endogenous | Rabbit IgG |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3969:
- Immunofluorescence
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 555 Conjugate) is not expected to recognize any specific antigen. It functions as an isotype control for rabbit monoclonal antibodies.
Source / Purification
IF-IC
Confocal immunofluorescent analysis of HT-29 cells using β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 555 Conjugate) (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology (CST) antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells.
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 3900 Rabbit (DA1E) mAb IgG XP® Isotype Control
- 2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
- 2985 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)Secondary Antibody250µlIF-IC HCA4414SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4416SAnti-rat IgG (H+L), (Alexa Fluor® 488 Conjugate)Secondary
XXXX原代细胞干预培养与激光共聚焦蛋白细胞定位实验 实验试剂：PBS(磷酸盐缓冲液pH7.4)（实验室常规配置）；非免疫山羊血清；Triton X-100（sigma）；BSA抗体稀释液（实验室常规配置）；A蛋白抗体（Santa cruz）；B蛋白抗体（Abcam）；Alexa Fluor® 488 donkey anti-goat IgG (H+L)(Invitrogen)；Alexa Fluor® 555 Anti-Rabbit IgG (H+L), F(ab
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
) directed against cell surface antigens of interest (biotinylated or labeled with an appropriate fluorochrome), and isotype-matched controls The protocol described here uses one biotinylated mAb in combination with streptavidin-Alexa Fluor 488
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