- 询价
- Cell Signaling Technology
- 8897
- USA
- 2025年07月12日
- W, IP
- Rabbit
- H,M,R,Mk,B,Dg,Pg,GP,Hr
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- 详细信息
- 技术资料
- 抗体英文名：
Derlin-1 Antibody
- 抗原：
synthetic peptide corresponding to residues near the carboxy terminus of human Derlin-1 protein
- 应用范围：
W, IP
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
H,M,R,Mk,B,Dg,Pg,GP,Hr
- 库存：
大量
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine Dg=Dog Pg=Pig GP=Guinea Pig Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk (B) (Dg) (Pg) (GP) (Hr) | Endogenous | 22 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Derlin-1 Antibody recognizes endogenous levels of total Derlin-1 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with either Derlin-2 or Derlin-3. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Derlin-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human Derlin-1 (hDerlin-1-Myc/DDK; +), using Derlin-1 Antibody. Western Blotting
Western blot analysis of various cell line and tissue extracts using Derlin-1 Antibody. |
| Background | Elimination of misfolded proteins from the endoplasmic reticulum (ER) occurs largely through the ER-associated degradation (ERAD) pathway and is an important physiological adaptation to ER stress. After insertion into the lumen of the ER, glycoproteins that fail to fold properly are destined for degradation. Through a process termed retro-translocation, misfolded proteins are deposited into the cytosol from the ER, where ubiquitination, deglycosylation, and proteasomal proteolysis lead to their degradation. Derlin-1 (Der1-like protein) corresponds to a homologue of yeast Der1p, a protein identified in a genetic screen for components required for the degradation of misfolded ER luminal proteins (1). Like yeast Der1p, mammalian Derlin-1 is an ER protein that is predicted to have four transmembrane segments with both the amino and carboxy termini exposed to the cytoplasmic compartment (2-4). Derlin-1 appears to be a central, evolutionarily conserved membrane component of the retro-translocation machinery associated with the ERAD pathway. Indeed, studies have shown that Derlin-1 expression is transcriptionally upregulated in response to ER stress (5-7) and associates with ER-anchored ubiquitin ligases, such as HRD1 and gp78/AMFR, via binding to p97/VCP and VCP-interacting membrane protein (VIMP) (5,8).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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