- 询价
- Cell Signaling Technology
- 8861
- USA
- 2025年07月14日
- W
- Rabbit
- H,M,R,Mk,Hm,Dg
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 抗体英文名：
Phospho-MCM2 (Ser139) Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Ser139 of human MCM2 protein
- 应用范围：
W
- 宿主：
Rabbit
- 适应物种：
H,M,R,Mk,Hm,Dg
- 级别：
详见MSDS文件
- 库存：
大量
- 供应商：
CST
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk (Hm) (Dg) | Endogenous | 125 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-MCM2 (Ser139) Antibody recognizes endogenous levels of MCM2 protein only when phosphorylated at Ser139. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser139 of human MCM2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from WI-38 cells, synchronized by serum starvation, using Phospho-MCM2 (Ser139) Antibody (upper), MCM2 (D7G11) XP® Rabbit mAb #3619 (middle), and β-Actin (13E5) Rabbit mAb #4970 (lower). Cells were synchronized for 72 hours and then released by addition of serum and harvested at the times indicated. Western Blotting
Western blot analysis of extracts from U-2 OS cells, untreated (-) or treated with calf intestinal phosphatase (CIP) and λ phosphatase (+), using Phospho-MCM2 (Ser139) Antibody (upper) and MCM2 (D7G11) XP® Rabbit mAb #3619 (lower). |
| Background | The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
