- 询价
- Cell Signaling Technology
- 5420
- USA
- 2025年10月17日
- W, IP, IF-IC
- Rabbit
- H,M,Mk
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- 详细信息
- 技术资料
- 抗体英文名：
SPARC Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Gly53 of human SPARC
- 应用范围：
W, IP, IF-IC
- 宿主：
Rabbit
- 保质期：
详见说明书
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 适应物种：
H,M,Mk
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H M Mk | Endogenous | 42 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | SPARC Antibody detects endogenous levels of total SPARC protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly53 of human SPARC. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of various cell extracts using SPARC Antibody. Jurkat are negative, as expected. IF-IC
Confocal immunofluorescent analysis of NTERA-2 (left) and SW620 (right) cells using SPARC Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | SPARC (secreted protein acidic and rich in cysteine), also known as osteonectin and BM40, is a secreted matricellular glycoprotein that belongs to a group of functionally related glycoproteins that includes tenascins C and X, thrombospondins 1 and 2, and osteopontin (1). Members in this class of glycoproteins are involved in tissue renewal, tissue remodeling, and embryonic development and work by exerting counter-adhesive and antiproliferative effects that lead to changes in cell shape, disruption of cell adhesion, and inhibition of the cell cycle (2). SPARC is expressed at high levels in bone tissue but is widely distributed in many other tissues and cell types (3), and is known to be associated with tissues undergoing morphogenesis, angiogenesis, mineralization, and other pathological responses to injury and tumorigenesis (4,5). SPARC has also been linked with obesity and diabetes (6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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