- 询价
- Cell Signaling Technology
- 5753
- USA
- 2025年11月19日
- W, IP
- H,M,R,Mk
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser206 of human Smad1 protein
- 应用范围：
W, IP
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
H,M,R,Mk
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H (M) (R) (Mk) | Endogenous | 60 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb recognizes endogenous levels of Smad1 protein only when phosphorylated at Ser206. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser206 of human Smad1 protein. Western Blotting
Western blot analysis of extracts of HeLa cells, untreated or UV-treated (60 mJ/cm2 for 2 minutes followed by 1.5 hour recovery), using Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb (upper) and Smad1 Antibody #9743 (lower). Western Blotting
Western blot analysis of extracts from HT-1080 cells, untreated or treated with TPA #4174 (200 nM for 30 minutes), using Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb (upper) and Smad1 Antibody #9743 (lower). |
| Background | Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).MAP kinases and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptional action by recruiting YAP to the linker region (7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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