- 询价
- Cell Signaling Technology
- 8657
- USA
- 2025年11月16日
- W
- H,M,R,Mk,Dg,Pg,GP
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
PA28α (D4F12) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues near the amino terminus of human PA28α protein
- 应用范围：
W
- 供应商：
CST
- 适应物种：
H,M,R,Mk,Dg,Pg,GP
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dg=Dog Pg=Pig GP=Guinea Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk (Dg) (Pg) (GP) | Endogenous | 28 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | PA28α (D4F12) Rabbit mAb recognizes endogenous levels of total PA28α protein. This antibody does not cross-react with either PA28β/PSME2 or PA28γ/PSME3 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PA28α protein. Western Blotting
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with Myc/DDK-tagged cDNA expression constructs encoding full-length human PA28α (hPA28α; +), full-length human PA28β (hPA28β; +), or full-length human PA28γ (hPA28γ; +), using PA28α (D4F12) Rabbit mAb (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower). Western Blotting
Western blot analysis of extracts from various cell lines using PA28α (D4F12) Rabbit mAb. |
| Background | The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. There are three evolutionarily conserved subunits of PA28: PA28α (PSME1), PA28β (PSME2), and PA28γ (PSME3) (1,2). PA28α and PA28β form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s). The PA28α/β complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptides from foreign proteins (2). PA28γ exists in the form of a homoheptamer and is mainly located in the nucleus. The PA28γ complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation (3,4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Isolation and Purification of Rabbit Osteoclasts
rabbit osteoclasts on resorbable substrates in vitro are an ideal model of osteoclast behaviour in vivo, and for some studies may be preferable to osteoclast-like cells generated in vitro from bone marrow cultures or from human peripheral blood
家兔（Rabbit,Oryctolagus curiculus）
家兔（Rabbit,Oryctolagus curiculus） 一、生物学特性和解剖生理特点 1．家兔属于哺乳纲、啮齿目、兔科、草食性哺乳动性。 2．家兔体小力弱、胆小怕惊、怕热、怕潮，喜欢安静、清洁、干燥、凉爽的环境，不能忍受污秽的条件。 3．是食草类（Herbivorous）单胃动物。饲养原则是以青粗食米为主，精饲料为辅。喜欢独居，白天活动少，都处于假眠或休息状态，夜间活动大，吃食多。有啃木、扒土的习惯
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