- 询价
- Cell Signaling Technology
- 5686
- USA
- 2025年11月14日
- W
- H,M,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
PPP2R4 (5G3) Mouse mAb
- 抗原：
recombinant protein specific to the mouse PPP2R4 protein
- 应用范围：
W
- 供应商：
CST
- 级别：
详见MSDS文件
- 适应物种：
H,M,Mk
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M Mk | Endogenous | 40 | Mouse IgG2a |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | PPP2R4 (5G3) Mouse mAb recognizes endogenous levels of total PPP2R4 protein. This antibody does not cross-react with other PP2A proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the mouse PPP2R4 protein. Western Blotting
Western blot analysis of extracts from MCF7, Hep G2, and HT-29 cells using PPP2R4 (5G3) Mouse mAb. |
| Background | Serine/threonine protein phosphatase 2A regulatory subunit 4 (PPP2R4) is one of the constituents of the regulatory subunit B' of PP2A (1). PPP2R4 is also known as the phosphatase activator (PTPA) and known to have the peptidyl prolyl isomerase (PPIase) activity (2-3). PPIases catalyze cis-trans isomerization of peptide bonds of proline residues in the polypeptide chain; this leads to the rapid folding of proteins (2). The ATPase activity of PPP2R4 is required for the phosphotyrosyl phosphatase activity of PP2A (2-4). Evidence suggests that PPP2R4 induces apoptosis independent of the canonical PP2A-mediated pathways (4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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