- 询价
- Cell Signaling Technology
- 5664
- USA
- 2026年05月28日
- western blot,免疫沉淀（IP）,免疫荧光（IF）
- 兔
- 人,小鼠,大鼠
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
AJE 润色服务限时 8 折起，权威机构信任 15+ 年Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
CNPase (D83E10) XP Rabbit mAb
- 抗原：
/
- 应用范围：
western blot,免疫沉淀（IP）,免疫荧光（IF）
- 宿主：
兔
- 适应物种：
人,小鼠,大鼠
- 抗原来源：
/
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Neuroscience
CNPase (D83E10) XP® Rabbit mAb #5664
PhosphoSitePlus ® protein, site, and accession data: CNP
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-F | H M R | Endogenous | 47 | Rabbit IgG |
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
CNPase (D83E10) XP® Rabbit mAb recognizes endogenous levels of total CNPase protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val81 of human CNPase protein.
Western Blotting
Western blot analysis of extracts from various brain tissues using CNPase (D83E10) XP® Rabbit mAb.
IF-F
Confocal immunofluorescent analysis of rat cerebellum using CNPase (D83E10) XP® Rabbit mAb (green) and α/β-Synuclein (Syn205) Mouse mAb #2644 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
CNPase (2', 3’-cyclic nucleotide 3'-phosphodiesterase) catalyzes the in vitro hydrolysis of 2’, 3’-cyclic nucleotides to produce 2’-nucleotides. The in vivo molecular function and native substrate of this nucleotide phosphodiesterase remains under investigation (1). High CNPase expression is seen in oligodendrocytes and Schwann cells as CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (2). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (3). Typical myelination is seen in CNPase knock-out mice, but they suffer severe neurodegeneration from axonal loss and oligodendrocytes display abnormal paranodal loop structure prior to axonal degeneration. Paranodal loops typically contact the axolemma in axon-glial signaling; neurodegeneration in CNPase knock-out mice is a secondary consequence of impaired cell-cell communication (4).
- Esposito, C. et al. (2008) Biochemistry 47, 308-19.
- Kozlov, G. et al. (2003) J Biol Chem 278, 46021-8.
- Lee, J. et al. (2005) J Cell Biol 170, 661-73.
- Lappe-Siefke, C. et al. (2003) Nat Genet 33, 366-74.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 2301 Phospho-DARPP-32 (Thr75) Antibody
- 2835 Neurofilament-L (DA2) Mouse mAb
- 3381 Phospho-NMDAR1 (Ser890) Antibody
- 4542 MAP2 Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 2154 Musashi Antibody
- 3670 GFAP (GA5) Mouse mAb
- 2838 Neurofilament-M (RMO 14.9) Mouse mAb
- 2507 PSD95 Antibody
- 2836 Neurofilament-H (RMdO 20) Mouse mAb
- 4604 Doublecortin Antibody
- 2306 DARPP-32 (19A3) Rabbit mAb
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 3877 Phospho-Vimentin (Ser56) Antibody
- 5393 Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb
- 2837 Neurofilament-L (C28E10) Rabbit mAb
XP® and Cell Signaling Technology® are trademarks of Cell Signaling Technology, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
肺癌病人的诊断和个性化治疗提供了有效的筛查工具。同样，以ROS1融合蛋白抗体（克隆号D4D6，货号#3287）为基础的IHC检测技术与断裂FISH方法相比，在非小细胞肺癌上IHC检测的准确度达到100%【5】。如此，我们期待检测ROS1融合变异的临床诊断用IHC试剂盒不久将成功推出。如要了解更多有关ALK和ROS1抗体的验证数据，请点击：ALK (D5F3) XP®Rabbit mAb #3633 和 ROS1 (D4D6) Rabbit mAb #3287。 本文
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
技术资料暂无技术资料 索取技术资料
