Phospho-FoxO3a (Ser294) Antibo

5538:

Immunoprecipitation , Western Blotting

Phospho-FoxO3a (Ser294) Antibody detects exogenous and endogenous levels of FoxO3a protein only when phosphorylated at serine 294.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Ser294 of human FoxO3a. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from 293T cells, transfected with tagged FoxO3a, using Phospho-FoxO3a (Ser294) Antibody.

Western Blotting

Western blot analysis of extracts from C2C12 cells, untreated or H₂ O₂ treated, using Phospho-FoxO3a (Ser294) Antibody (upper) or FoxO3a (75D8) Rabbit mAb #2497 (lower).

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).

Erk phosphorylates FoxO3a at Ser294, Ser344 and Ser425, resulting in degradation of FoxO3a through the MDM2-mediated ubiquitin-proteasome pathway. Thus, Erk promotes proliferation and tumor progression by inhibiting FoxO3a (10).

1. Anderson, M.J. et al. (1998) Genomics 47, 187-199.
2. Galili, N. et al. (1993) Nat. Genet. 5, 230-235.
3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
4. Nakae, J. et al. (1999) J. Biol. Chem. 274, 15982-15985.
5. Rena, G. et al. (1999) J. Biol. Chem. 274, 17179-17183.
6. Guo, S. et al. (1999) J. Biol. Chem. 274, 17184-17192.
7. Seoane, J. et al. (2004) Cell 117, 211-223.
8. Arden, K.C. (2004) Mol. Cell 14, 416-418.
9. Yang, Y. et al. (2005) EMBO J. 24, 1021-1032.
10. Yang, J.Y. et al. (2008) Nat Cell Biol 10, 138-48.

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• 2497 FoxO3a (75D8) Rabbit mAb
• 2880 FoxO1 (C29H4) Rabbit mAb
• 9946 Forkhead Signaling Antibody Sampler Kit
• 6302 SignalSilence^® FoxO3a siRNA I
• 6303 SignalSilence^® FoxO3a siRNA II
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
• 9998 BSA
• 9999 Nonfat Dry Milk

For Research Use Only. Not For Use In Diagnostic Procedures.