- 询价
- Cell Signaling Technology
- 9108
- USA
- 2025年08月26日
- W
- Rabbit
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
p42 MAP Kinase (Erk2) Antibody
- 抗原：
synthetic peptide corresponding to human p42 MAP kinase (Erk2)
- 应用范围：
W
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 42 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | p42 MAP Kinase (Erk2) Antibody detects endogenous levels of p42 MAP kinase (Erk2). The antibody does not recognize p38 MAP kinase, p44 MAP kinase (Erk1) or SAPK/JNK. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human p42 MAP kinase (Erk2). Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Pool p42 MAPK siRNA or single-duplex SignalSilence® p42 MAPK siRNA (+), using p42 MAP Kinase (Erk2) Antibody #9108 and α-Tubulin (11H10) Rabbit mAb #2125. The p42 MAP Kinase (Erk2) Antibody confirms silencing of p42 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p42 siRNA. Western Blotting
Western blot analysis of extracts from various cell lines, using p42 MAP Kinase (Erk2) Antibody. |
| Background | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Reconstitution of the Nuclear Transport of the MAP Kinase ERK2
to be CRM1- and MEK1/2-dependent. Here, we describe a general nuclear import assay of wild-type ERK2 that can be employed to identify different mechanisms governing nuclear entry of the protein kinase, adapted to evaluate ERK2 mutants that impair nuclear
Direct Kinase Assay Screening for Inhibitors of MAP Kinase
An important convergence point involved in the signal transduction pathways of many different growth factors, hormones, and cytokines are the p42 and p44 serine/ threonine kinases called either MAP kinases (mitogen-activate protein kinases
. We have shown that MAP kinase ERK2 is activated via the cAMP receptor CAR1 in phase with this periodic change. This was revealed by assessing the level of ERK2 activation with immunoblots using two kinds of antibodies, commercially available anti-phospho-p44/p42
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