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Enolase-2 (D20H2) Rabbit mAb

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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2025年10月24日
  • W, IP
  • H,M,R,Mk
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体英文名

      Enolase-2 (D20H2) Rabbit mAb

    • 抗原

      synthetic peptide corresponding to residues near the carboxy terminus of human enolase-2 protein

    • 应用范围

      W, IP

    • 库存

      大量

    • 保质期

      详见说明书

    • 适应物种

      H,M,R,Mk

    • 级别

      详见MSDS文件

    • 供应商

      CST

    • 是否单克隆

      1

    • 保存条件

      -20°c

    • 规格

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting  IP=Immunoprecipitation
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W IP H M R Mk Endogenous 47 Rabbit IgG
    Protocols
    Specificity / Sensitivity

    Enolase-2 (D20H2) Rabbit mAb recognizes endogenous levels of total enolase-2 protein.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human enolase-2 protein.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from various cell lines using Enolase-2 (D20H2) Rabbit mAb.

    Background

    Enolase is a glycolytic enzyme that is involved in the conversion of 2-phosphoglycerate to phosphoenolpyruvate (1). Mammalian enolase has three subunits: α, β, and γ, that can form homo and heterodimers. Homodimers of γ enolase are neuronal-specific (2). Research studies have shown elevated levels of neuro-specific enolase-2 in neuroblastoma (2) and small-cell lung cancer (3,4).

    1. Van Obberghen, E. et al. (1988) J Neurosci Res 19, 450-6.
    2. Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
    3. Stern, P. et al. (2007) Tumour Biol 28, 84-92.
    4. O'Shea, P. et al. (1995) Ir J Med Sci 164, 31-6.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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    图标文献和实验
    相关实验
    • Purification of mAb (IgG)

      pellets in 10 ml of 100 mM Tris buffer or Binding buffer. And pool into 50 ml Falcon tubes (try not to make subbles).Use 3.2 ml/cm 12-14,000 MW dialysis tubing. Heat tubing in 500 ml H2O in microwave. Not boiling. Rinse tubing in fresh H2O several times

    • Purification of mAb (IgG)

        Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers

    • 组化染色背景高?没信号?一篇文章带你快速掌握免疫组化!

      :使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫

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