Phospho-MARCKS (Ser152/156) An

2741:

Western Blotting

Phospho-MARCKS (Ser152/156) Antibody detects endogenous levels of MARCKS only when phosphorylated at serines 152 and 156 .

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser152/156 of rat MARCKS. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from NIH/3T3 and C6 cells starved for 16 hours and treated with TPA (0.2 µM) for 30 minutes.

M yristoylated A lanine-R ich C -K inase S ubstrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress, which regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).

1. Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
2. Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
3. Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
4. Hartwig, J.H. et al. (1992) Nature 356, 618-22.
5. Thelen, M. et al. (1991) Nature 351, 320-2.

• Iwabu, A. et al. (2004) Epidermal growth factor induces fibroblast contractility and motility via a protein kinase C delta-dependent pathway. J. Biol. Chem. 279, 14551-14560. Applications: Western Blotting
• Volmer, R. et al. (2006) PLoS Pathog 2, e19. Applications: Western Blotting
• Park, JA. et al. (2005) Human neutrophil elastase induces hypersecretion of mucin from well-differentiated human bronchial epithelial cells in vitro via a protein kinase C{delta}-mediated mechanism. Am J Pathol. 2005 Sep;167(3):651-61. Applications: Western Blotting

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For Research Use Only. Not For Use In Diagnostic Procedures.