- 询价
- Cell Signaling Technology
- 5354
- USA
- 2025年09月09日
- W
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (Biotinylated)
- 抗原：
synthetic peptide corresponding to L858R mutant sequence of human EGF receptor
- 应用范围：
W
- 级别：
详见MSDS文件
- 适应物种：
H
- 供应商：
CST
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Endogenous | 175 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | EGFR (L858R Mutant Specific) (43B2) Rabbit mAb (Biotinylated) detects endogenous levels of EGFR mutant L858R protein. Careful titration of this antibody may be required to obtain optimal specificity. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to L858R mutant sequence of human EGF receptor. Western Blotting
Western blot analysis of extracts from HCC827 and H3255 cells using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (Biotinylated) and detected with Strepavidin-HRP #3999. |
| Description | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated EGFR (L858R Mutant Specific) (43B2) Rabbit mAb #3197. |
| Background | The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Overview of Receptor Allosterism
, L., Valant, C., Flynn, B.L., Sexton, P.M., Christopoulos, A., and Scammells, P.J. 2009. Allosteric modulators of the adenosine A1 receptor: Synthesis and pharmacological evaluation of 4‐substituted 2‐amino‐3‐benzoylthiophenes. J. Med. Chem. 52:4543
Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
receptor. Nature 389:753‐758. Glickman, J.F., Wu, X., Mercuri, R., Illy, C., Bowen, B.R., He, Y., and Sills, M. 2002. A comparison of ALPHAScreen, TR‐FRET, and TRF as assay
Blocking of unwanted non-specific staining
and other cell types. By exploiting the preferential avidity of Fc receptor for human > mouse Ig> rabbit > swine > goat immunoglubulins, one may use a blocking of the receptors with a reagent which will not interfere with the secondary reagents
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