- 询价
- Cell Signaling Technology
- 2038
- USA
- 2025年09月05日
- W, IP, IHC-P, IF-IC, F
- Rabbit
- H,M,R,Mk,Dm,C,Pg
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
PP2A C Subunit Antibody
- 抗原：
synthetic peptide corresponding to amino acids at the carboxy terminus of human PP2A catalytic subunit
- 应用范围：
W, IP, IHC-P, IF-IC, F
- 宿主：
Rabbit
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk,Dm,C,Pg
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken Dm=D. melanogaster Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R Mk Dm (C) (Pg) | Endogenous | 36, 38 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | This antibody detects endogenous levels of PP2A catalytic subunit protein (both α and β isoforms). The antibody does not cross-react with other PP2A subunits. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the carboxy terminus of human PP2A catalytic subunit. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells using PP2A C Subunit Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PP2A C Subunit Anitbody in the presence of control peptide (left) or PP2A C Subunit Blocking Peptide, #1067 (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using PP2A C Subunit Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PP2A C Subunit Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PP2A C Subunit Antibody in the presence of control peptide (left) or antigen-specific peptide, #1067 (right). Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using PP2A C Subunit Antibody (blue) compared to a non-specifc negative control antibody (red). IF-IC
Immunofluorescent analysis of paraformaldehyde fixed HeLa cells using PP2A C Subunit Antibody. |
| Background | Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B'' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
Twenty-five years ago, Georges K�hler and C�sar Milstein invented a means of cloning individual antibodies, thus opening up the way for tremendous advances in the fields of cell biology and clinical diagnostics ( 1 ). However, in spite
volumes running buffer (the serum albumin should stick to the column and the Ig should flow through).(6) On ice slowly add saturated ammonium sulfate to 45% (550 ml sample 450 ml saturated ammonium sulfate). Tilt overnight at 4°C.(7) Pellet by spinning
has eluted into the column. c) Add 5mL of PBS on top. d)Open Stopcock. collect in 2mL fractions, discarding the first 2mL and keeping the 2nd 2mL fraction. The second 2mL fraction contains the conjugated antibody*. *NOTE: Step d) appropriate only if 1mL
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