- 询价
- Cell Signaling Technology
- 8138
- USA
- 2025年10月22日
- W, IP, IF-F
- R,H,M
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Insulin (L6B10) Mouse mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Val36 of human insulin
- 应用范围：
W, IP, IF-F
- 级别：
详见MSDS文件
- 供应商：
CST
- 保质期：
详见说明书
- 库存：
大量
- 适应物种：
R,H,M
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-F | R (H) (M) | Endogenous | 6 | Mouse IgG2a |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Insulin (L6B10) Mouse mAb recognizes endogenous levels of total insulin and proinsulin. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val36 of human insulin. Western Blotting
Western blot analysis of extracts from INS-1 cells using Insulin (L6B10) Mouse mAb. IF-F
Confocal immunofluorescent analysis of rat pancreas using Insulin (L6B10) Mouse mAb (green) and S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Assessment of Insulin Secretion in the Mouse
. 1 ). The mouse synthesizes two molecular forms of insulin and C-peptide, which are identical to their respective rat counterparts ( 5 ). The two rodent insulins, designated insulin I and II, are present at a ratio of 1∶3 in the mouse and 4∶1 in the rat
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
A Mouse Model of Diet-Induced Obesity and Insulin Resistance
-of-function mutations, polygenic models, and different environmental exposure models. The mouse model of diet-induced obesity has become one of the most important tools for understanding the interplay of high-fat Western diets and the development of obesity
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