- 询价
- Cell Signaling Technology
- 5246
- USA
- 2026年05月28日
- W, IP, IHC-P, IF-IC, ChIP
- H,M,R,Mk
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- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
Ezh2 (D2C9) XP Rabbit mAb__®
- 抗原：
synthetic peptide corresponding to residues surrounding Arg354 of human Ezh2 protein
- 应用范围：
W, IP, IHC-P, IF-IC, ChIP
- 供应商：
CST
- 适应物种：
H,M,R,Mk
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC ChIP | H M R Mk | Endogenous | 98 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Ezh2 (D2C9) XP® Rabbit mAb detects endogenous levels of total Ezh2 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg354 of human Ezh2 protein. Western Blotting
Western blot analysis of extracts from MCF7, Neuro-2a, and COS-7 cell lines using Ezh2 (D2C9) XP® Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ezh2 (D2C9) XP® Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using Ezh2 (D2C9) XP® Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lymphoma using Ezh2 (D2C9) XP® Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded 4T1a mouse syngeneic tumor using Ezh2 (D2C9) XP® Rabbit mAb. IF-IC
Confocal immunofluorescent analysis of HeLa cells using Ezh2 (D2C9) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (blue). Actin filaments were labeled with DY-554 phalloidin (red). Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 5 µl of Ezh2 (D2C9) XP® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
| Background | The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro , which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
实际上将作为阴性对照使用。CST 推荐使用 Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) #4620。该抗体可检测与基因组中所有 DNA 序列结合的所有组蛋白 H3 类型（H3、H3.3、CENP-A）。因此，不管检测的位点活性状态如何，该抗体都能为 ChIP 实验提供通用的阳性对照。 阴性对照 阴性对照抗体（如正常兔 IgG）不能识别特异抗原表位，因此可用于检测非特异性结合。例如，如果阴性对照样品中的产物量等于特异
Using Soil DNA Kit (D5625) to Clean Up DNA containing inhibitors
) and incubate for 4 minutes. Centrifuge at max speed for 30 seconds and discard flowthrough. 7. Add equal volume of XP2 buffer to the cleared lysate, mix the sample throughly by vortexing. 8. Apply entire sample including any precipitation
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