- 询价
- Cell Signaling Technology
- 4466
- USA
- 2026年05月28日
- W, IHC-P, IF-F
- H,M,R
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- 文献和实验
- 技术资料
- 抗体英文名：
β3-Tubulin (TU-20) Mouse mAb
- 抗原：
synthetic peptide corresponding to the carboxy terminus of human β3-tubulin protein
- 应用范围：
W, IHC-P, IF-F
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
H,M,R
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-F | H M R | Endogenous | 55 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | β3-Tubulin (TU-20) Mouse mAb detects endogenous levels of total β3-tubulin protein and does not cross-react with tubulin isoforms expressed in non-neuronal cells. This clone is similar to TUJ1. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human β3-tubulin protein. Western Blotting
Western blot analysis of extracts from fetal rat and neonatal mouse brain using β3-Tubulin (TU-20) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat brain using beta3-Tubulin (TU-20) Mouse mAb. IF-F
Confocal immunofluorescent image of rat cerebellum labeled with β3-Tubulin (TU-20) Mouse mAb (green) and Neurofilament-L (C28E10) Rabbit mAb #2837 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
人整合素αVβ3（ITG αⅤβ3）酶联免疫分析（ELISA）
人整合素αVβ3（ITG αⅤβ3） 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 整合素αVβ3（ITG αⅤβ3） 的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 人 整合素αV β 3 （ ITG αⅤβ 3 ） 水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 整合素αV β 3 （ ITG αⅤ
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