Mouse IL-1R1 / CD121a ELISA Pair Set | 小鼠 IL-1R1 / CD121a 酶联免疫配对抗体组合

Mouse IL-1R1 / CD121a ELISA Pair Set（产品说明）
反应种属：Mouse
试剂对靶点：IL1R1
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：62.5-4000
试剂对标记类型：HRP
包被抗体：0.4 mg/mL of rabbit anti-Mouse IL-1R1 / CD121a monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50807-R002)
检测抗体：0.2 mg/mL of rabbit anti-Mouse IL-1R1 / CD121a polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
试剂灵敏度：The minimum detectable dose of Mouse IL-1R1 / CD121a was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Mouse IL-1R1 / CD121a ELISA Pair Set is for the quantitative determination of Mouse IL-1R1 / CD121a. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Mouse IL-1R1 / CD121a coated on a 96-well plate. Standards and samples are added to the wells, and any Mouse IL-1R1 / CD121a present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Mouse IL-1R1 / CD121a polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Mouse IL-1R1 / CD121a present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：62.5 pg/ml