- ￥2800 - 6800
- Sino Biological
- 北京
- SEK11102
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human IL18R1 / CD218a ELISA Pair Set
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2800.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥6800.0 |
Human IL18R1 / CD218a ELISA Pair Set（产品说明）
反应种属：Human
试剂对靶点：IL18R1
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：7.81-500
试剂对标记类型：HRP
包被抗体：1.0 mg/mL of rabbit anti-IL18R1 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 11102-R011)
检测抗体：0.5 mg/mL mouse anti-IL18R1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11102-MM05)
试剂灵敏度：The minimum detectable dose of human IL18R1 was determined to be approximately 7.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理： The human IL18R1 / CD218a ELISA Pair Set is for the quantitative determination of human IL18R1. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL18R1 /CD218a coated on a 96-well plate. Standards and samples are added to the wells,and any IL18R1 / CD218a present binds to the immobilized antibody. The wellsare washed and a horseradish peroxidase conjugated mouse anti-IL18R1 / CD218amonoclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of IL18R1 / CD218a present inthe sample. To end the enzyme reaction, the stop solution is added andabsorbances of the microwell are read at 450 nm.
试剂对检测限：7.8 pg/ml
反应种属：Human
试剂对靶点：IL18R1
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：7.81-500
试剂对标记类型：HRP
包被抗体：1.0 mg/mL of rabbit anti-IL18R1 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 11102-R011)
检测抗体：0.5 mg/mL mouse anti-IL18R1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11102-MM05)
试剂灵敏度：The minimum detectable dose of human IL18R1 was determined to be approximately 7.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理： The human IL18R1 / CD218a ELISA Pair Set is for the quantitative determination of human IL18R1. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL18R1 /CD218a coated on a 96-well plate. Standards and samples are added to the wells,and any IL18R1 / CD218a present binds to the immobilized antibody. The wellsare washed and a horseradish peroxidase conjugated mouse anti-IL18R1 / CD218amonoclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of IL18R1 / CD218a present inthe sample. To end the enzyme reaction, the stop solution is added andabsorbances of the microwell are read at 450 nm.
试剂对检测限：7.8 pg/ml
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文献和实验该产品被引用文献
1, Wedervang-Resell, K; et al. Increased interleukin 18 activity in adolescents with early-onset psychosis is associated with cortisol and depressive symptoms. Psychoneuroendocrinology, Pubmed: 31761332
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Human IL18R1 / CD218a ELISA Pair Set | 人 IL18R1 / CD218a 酶联免疫配对抗体组合
￥2800 - 6800
